제품 > 항체 > 다중클론항체(pAb)

TMEM74 Rabbit pAb (A10379)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - TMEM74 Rabbit pAb (A10379)

Western blot analysis of extracts of DU145 cells, using TMEM74 antibody (A10379) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameTMEM74 Rabbit pAb
Catalog No.A10379
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Involved in macroautophagy. Predicted to be located in autophagosome membrane; cytoplasmic vesicle; and lysosomal membrane. Predicted to be integral component of membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TMEM74 (NP_694560.1).
SequenceMELHYLAKKSNQADLCDARDWSSRGLPGDQADTAATRAALCCQKQCASTPRATEMEGSKLSSSPASPSSSLQNSTLQPDAFPPGLLHSGNNQITAERKVCNCCSQELETSFTYVDKNINLEQRNRSSPSAKGHNHPGELGWENPNEWSQEAAISLISEEEDDTSSEATSSGKSIDYGFIS
Gene ID
Swiss Prot
SynonymsNET36
Calculated MW33kDa
Observed MW37kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesDU145
Cellular locationCytoplasmic vesicle, Lysosome membrane, Multi-pass membrane protein, autophagosome membrane
    ABclonal:Western blot - TMEM74 Rabbit pAb (A10379)}

    Western blot - TMEM74 Rabbit pAb (A10379)

    Western blot analysis of extracts of DU145 cells, using TMEM74 antibody (A10379) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.