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TOMM7 Rabbit pAb (A17711)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - TOMM7 Rabbit pAb (A17711)

Western blot analysis of lysates from 293F cells, using TOMM7 Rabbit pAb (A17711) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product nameTOMM7 Rabbit pAb
Catalog No.A17711
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a subunit of the translocase of the outer mitochondrial membrane. The encoded protein regulates the assembly and stability of the translocase complex.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-55 of human TOMM7 (NP_061932.1).
SequenceMVKLSKEAKQRLQQLFKGSQFAIRWGFIPLVIYLGFKRGADPGMPEPTVLSLLWG
Gene ID
Swiss Prot
SynonymsTOM7; TOMM7
Calculated MW6kDa
Observed MW11kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293F
Cellular locationmitochondrial outer membrane, mitochondrion.
Customer validation

WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - TOMM7 Rabbit pAb (A17711)}

    Western blot - TOMM7 Rabbit pAb (A17711)

    Western blot analysis of lysates from 293F cells, using TOMM7 Rabbit pAb (A17711) at 1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A17711? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (26)