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TPCN1 Rabbit pAb (A15847)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - TPCN1 Rabbit pAb (A15847)

Western blot analysis of lysates from rat lung, using TPCN1 Rabbit pAb (A15847) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product nameTPCN1 Rabbit pAb
Catalog No.A15847
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Voltage-gated Ca(2+) and Na+ channels have 4 homologous domains, each containing 6 transmembrane segments, S1 to S6. TPCN1 is similar to these channels, but it has only 2 domains containing S1 to S6 (Ishibashi et al., 2000 [PubMed 10753632]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 692-816 of human TPCN1 (NP_060371.2).
SequenceFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT
Gene ID
Swiss Prot
SynonymsTPC1; TPCN1
Calculated MW94kDa
Observed MW94kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRat lung
Cellular locationEndosome membrane, Lysosome membrane, Multi-pass membrane protein
Customer validation

IHC(Danio rerio)

ABclonal:Western blot - TPCN1 Rabbit pAb (A15847)}

Western blot - TPCN1 Rabbit pAb (A15847)

Western blot analysis of lysates from rat lung, using TPCN1 Rabbit pAb (A15847) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

* For research use only. Not for therapeutic or diagnostic purposes.

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