제품 > 항체 > 단일클론항체(mAb)

TPH-1 Rabbit mAb (A25610)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Immunohistochemistry - TPH-1 Rabbit mAb (A25610)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using TPH-1 Rabbit mAb (A25610) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TPH-1 Rabbit mAb (A25610)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using TPH-1 Rabbit mAb (A25610) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TPH-1 Rabbit mAb (A25610)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using TPH-1 Rabbit mAb (A25610) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TPH-1 Rabbit mAb (A25610)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using TPH-1 Rabbit mAb (A25610) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - TPH-1 Rabbit mAb (A25610)

Confocal imaging of paraffin-embedded Rat brain tissue using TPH-1 Rabbit mAb (A25610, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - TPH-1 Rabbit mAb (A25610)

Confocal imaging of paraffin-embedded Mouse brain tissue using TPH-1 Rabbit mAb (A25610, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining,

Overview

Product nameTPH-1 Rabbit mAb
Catalog No.A25610
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC65043
This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 102-402 of human TPH1 (NP_004170.1).
SequenceMETVPWFPKKISDLDHCANRVLMYGSELDADHPGFKDNVYRKRRKYFADLAMNYKHGDPIPKVEFTEEEIKTWGTVFQELNKLYPTHACREYLKNLPLLSKYCGYREDNIPQLEDVSNFLKERTGFSIRPVAGYLSPRDFLSGLAFRVFHCTQYVRHSSDPFYTPEPDTCHELLGHVPLLAEPSFAQFSQEIGLASLGASEEAVQKLATCYFFTVEFGLCKQDGQLRVFGAGLLSSISELKHALSGHAKVKPFDPKITCKQECLITTFQDVYFVSESFEDAKEKMREFTKTIKRPFGVKYN
Gene ID
Swiss Prot
SynonymsTPRH; TRPH
Calculated MW51kDa/53kDa
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IHC-P 1:200 - 1:800
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationImmunohistochemistry    Immunofluorescence    
Positive samples
Cellular locationcytosol, neuron projection.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Immunohistochemistry - TPH-1 Rabbit mAb (A25610)}

Immunohistochemistry - TPH-1 Rabbit mAb (A25610)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using TPH-1 Rabbit mAb (A25610) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TPH-1 Rabbit mAb (A25610)}

Immunohistochemistry - TPH-1 Rabbit mAb (A25610)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using TPH-1 Rabbit mAb (A25610) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TPH-1 Rabbit mAb (A25610)}

Immunohistochemistry - TPH-1 Rabbit mAb (A25610)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using TPH-1 Rabbit mAb (A25610) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TPH-1 Rabbit mAb (A25610)}

Immunohistochemistry - TPH-1 Rabbit mAb (A25610)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using TPH-1 Rabbit mAb (A25610) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - TPH-1 Rabbit mAb (A25610)}

Immunofluorescence - TPH-1 Rabbit mAb (A25610)

Confocal imaging of paraffin-embedded Rat brain tissue using TPH-1 Rabbit mAb (A25610, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - TPH-1 Rabbit mAb (A25610)}

Immunofluorescence - TPH-1 Rabbit mAb (A25610)

Confocal imaging of paraffin-embedded Mouse brain tissue using TPH-1 Rabbit mAb (A25610, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining,

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)