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TRA2B Rabbit pAb (A9580)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TRA2B Rabbit pAb (A9580)

Western blot analysis of various lysates, using TRA2B Rabbit pAb (A9580) at 1:900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunofluorescence - TRA2B Rabbit pAb (A9580)

Immunofluorescence analysis of HeLa cells using TRA2B Rabbit pAb (A9580) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - TRA2B Rabbit pAb (A9580)

Immunofluorescence analysis of PC-12 cells using TRA2B Rabbit pAb (A9580) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameTRA2B Rabbit pAb
Catalog No.A9580
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a nuclear protein which functions as sequence-specific serine/arginine splicing factor which plays a role in mRNA processing, splicing patterns, and gene expression. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human TRA2B (NP_004584.1).
SequenceMSDSGEQNYGERESRSASRSGSAHGSGKSARHTPARSRSKEDSRRSRSKSRSRSESRSRSRRSSRRHYTRSRSRSRSHRRSRSRSYSRDYRRRHSHSHSP
Gene ID
Swiss Prot
SynonymsSFRS10; SRFS10; TRAN2B; PPP1R156; TRA2-BETA; Htra2-beta; TRA2B
Calculated MW34kDa
Observed MW38kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMolt-4, 293F, Mouse brain
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - TRA2B Rabbit pAb (A9580)}

Western blot - TRA2B Rabbit pAb (A9580)

Western blot analysis of various lysates, using TRA2B Rabbit pAb (A9580) at 1:900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunofluorescence - TRA2B Rabbit pAb (A9580)}

Immunofluorescence - TRA2B Rabbit pAb (A9580)

Immunofluorescence analysis of HeLa cells using TRA2B Rabbit pAb (A9580) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TRA2B Rabbit pAb (A9580)}

Immunofluorescence - TRA2B Rabbit pAb (A9580)

Immunofluorescence analysis of PC-12 cells using TRA2B Rabbit pAb (A9580) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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