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TRAF3 Rabbit pAb (A3094)

Review (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TRAF3 Rabbit pAb (A3094)

Western blot analysis of extracts of various cell lines, using TRAF3 antibody (A3094) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - TRAF3 Rabbit pAb (A3094)

Immunofluorescence analysis of HeLa cells using TRAF3 antibody (A3094). Blue: DAPI for nuclear staining.

Review
ABclonal: review for TRAF3 Rabbit pAb(A3094)

Overview

Product nameTRAF3 Rabbit pAb
Catalog No.A3094
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from, members of the TNF receptor (TNFR) superfamily. This protein participates in the signal transduction of CD40, a TNFR family member important for the activation of the immune response. This protein is found to be a critical component of the lymphotoxin-beta receptor (LTbetaR) signaling complex, which induces NF-kappaB activation and cell death initiated by LTbeta ligation. Epstein-Barr virus encoded latent infection membrane protein-1 (LMP1) can interact with this and several other members of the TRAF family, which may be essential for the oncogenic effects of LMP1. The protein also plays a role in the regulation of antiviral response. Mutations in this are associated with Encephalopathy, acute, infection-induced, herpes-specific 5.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human TRAF3 (NP_003291.2).
SequenceMESSKKMDSPGALQTNPPLKLHTDRSAGTPVFVPEQGGYKEKFVKTVEDKYKCEKCHLVLCSPKQTECGHRFCESCMAALLSSSSPKCTACQESIVKDKV
Gene ID
Swiss Prot
SynonymsCAP1; LAP1; CAP-1; CRAF1; IIAE5; CD40bp; RNF118
Calculated MW64kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples22Rv1, SW480, MCF7, U-937, ES-2, Raji, Mouse gastrocnemius muscle, Mouse spleen
Cellular locationCytoplasm, Endosome, Mitochondrion
Customer validation

(HeLa)

Documents

Certificate of Compliance

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ABclonal:Western blot - TRAF3 Rabbit pAb (A3094)}

Western blot - TRAF3 Rabbit pAb (A3094)

Western blot analysis of extracts of various cell lines, using TRAF3 antibody (A3094) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - TRAF3 Rabbit pAb (A3094)}

Immunofluorescence - TRAF3 Rabbit pAb (A3094)

Immunofluorescence analysis of HeLa cells using TRAF3 antibody (A3094). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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