제품 > 항체 > 다중클론항체(pAb)

TRIM63 Rabbit pAb (A3101)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of 293F, using TRIM63 antibody (A3101) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Western blot analysis of Rat heart, using TRIM63 antibody (A3101) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - TRIM63 Rabbit pAb (A3101)

Immunofluorescence analysis of HeLa cells using TRIM63 Rabbit pAb (A3101) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	TRIM63 Rabbit pAb
Catalog No.	A3101
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 253-353 of human TRIM63 (NP_115977.2).
Sequence	LDKSTKLVETAIQSLDEPGGATFLLTAKQLIKSIVEASKGCQLGKTEQGFENMDFFTLDLEHIADALRAIDFGTDEEEEEFIEEEDQEEEESTEGKEEGHQ
Gene ID
Swiss Prot
Synonyms	IRF; SMRZ; MURF1; MURF2; RNF28
Calculated MW	40kDa
Observed MW	47kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	293F, Rat heart
Cellular location	Cytoplasm, M line, Nucleus, Z line, myofibril, sarcomere
Customer validation	WB(Sus scrofa, Homo sapiens, Mus musculus, Rattus norvegicus, Ochotona princeps) IF(Homo sapiens)

Documents

Certificate of Compliance

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Research Area

TRIM63 Rabbit pAb images

Western blot - TRIM63 Rabbit pAb (A3101)

Immunofluorescence - TRIM63 Rabbit pAb (A3101)

Immunofluorescence analysis of HeLa cells using TRIM63 Rabbit pAb (A3101) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for TRIM63 Rabbit pAb (A3101)

Product:

TRIM63 Rabbit pAb

Journal:

Animal bioscience

Application:

IF:

2.4

Species:

Sus scrofa

PMID:

34445847

Title:

Effects of protein content and the inclusion of protein sources with different amino acid release dynamics on the nitrogen utilization of weaned piglets

References for TRIM63 Rabbit pAb (A3101)

Product:

TRIM63 Rabbit pAb

Journal:

Molecular therapy. Nucleic acids

Application:

IF:

6.5

Species:

Homo sapiens

PMID:

36891498

Title:

Identification of a novel small-molecule inhibitor of miR-29b attenuates muscle atrophy - PMC

References for TRIM63 Rabbit pAb (A3101)

Product:

TRIM63 Rabbit pAb

Journal:

Chemico-biological interactions

Application:

IF:

4.7

Species:

Mus musculus

PMID:

37257577

Title:

AKR1B1 inhibition using NARI-29-an Epalrestat analogue-alleviates Doxorubicin-induced cardiotoxicity via modulating Calcium/CaMKII/MuRF-1 axis

References for TRIM63 Rabbit pAb (A3101)

Product:

TRIM63 Rabbit pAb

Journal:

Journal of the science of food and agriculture

Application:

IF:

4.1

Species:

Rattus norvegicus

PMID:

37160715

Title:

Oat glucan alleviates muscle atrophy via promoting myotube formation and suppressing protein degradation

References for TRIM63 Rabbit pAb (A3101)

Product:

TRIM63 Rabbit pAb

Journal:

BMC genomics

Application:

IF:

3.5

Species:

Mus musculus

PMID:

38331723

Title:

Uncovering the potential molecular mechanism of liraglutide to alleviate the effects of high glucose on myoblasts based on high-throughput transcriptome sequencing technique - PMC

References for TRIM63 Rabbit pAb (A3101)

Product:

TRIM63 Rabbit pAb

Journal:

Molecular and cellular endocrinology

Application:

WB, IF

IF:

3.8

Species:

Homo sapiens

PMID:

35390449

Title:

Tripartite motif containing 63, regulated by E26 transformation specific variant 4, facilitates the thyroid carcinoma progression and the AKT, p38, and ERK signaling pathways

References for TRIM63 Rabbit pAb (A3101)

Product:

TRIM63 Rabbit pAb

Journal:

BMC pulmonary medicine

Application:

IF:

2.6

Species:

Ochotona princeps

PMID:

38097957