제품 > 항체 > 다중클론항체(pAb)

TRRAP Rabbit pAb (A18849)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - TRRAP Rabbit pAb (A18849)

Western blot analysis of various lysates using TRRAP Rabbit pAb (A18849) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - TRRAP Rabbit pAb (A18849)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using TRRAP Rabbit pAb (A18849) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameTRRAP Rabbit pAb
Catalog No.A18849
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a large multidomain protein of the phosphoinositide 3-kinase-related kinases (PIKK) family. The encoded protein is a common component of many histone acetyltransferase (HAT) complexes and plays a role in transcription and DNA repair by recruiting HAT complexes to chromatin. Deregulation of this gene may play a role in several types of cancer including glioblastoma multiforme. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 3660-3859 of TRRAP (NP_001231509.1).
SequencePNATDYWTFRKMFTIQLALIGFAEFVLHLNRLNPEMLQIAQDTGKLNVAYFRFDINDATGDLDANRPVPFRLTPNISEFLTTIGVSGPLTASMIAVARCFAQPNFKVDGILKTVLRDEIIAWHKKTQEDTSSPLSAAGQPENMDSQQLVSLVQKAVTAIMTRLHNLAQFEGGESKVNTLVAAANSLDNLCRMDPAWHPWL
Gene ID
Swiss Prot
SynonymsTra1; TR-AP; DEDDFA; DFNA75; PAF400; STAF40; PAF350/400; TRRAP
Calculated MW438kDa
Observed MW434kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, Rat testis
Cellular locationGolgi apparatus, nucleoplasm, nucleus

Documents

Certificate of Compliance

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    ABclonal:Western blot - TRRAP Rabbit pAb (A18849)}

    Western blot - TRRAP Rabbit pAb (A18849)

    Western blot analysis of various lysates using TRRAP Rabbit pAb (A18849) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - TRRAP Rabbit pAb (A18849)}

    Immunohistochemistry - TRRAP Rabbit pAb (A18849)

    Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using TRRAP Rabbit pAb (A18849) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.