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TWIST1 Rabbit mAb (A25134)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TWIST1 Rabbit mAb (A25134)

Western blot analysis of various lysates using TWIST1 Rabbit mAb (A25134) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): K-562.
Exposure time: 90s.

ABclonal:Immunohistochemistry - TWIST1 Rabbit mAb (A25134)

Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using TWIST1 Rabbit mAb (A25134) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TWIST1 Rabbit mAb (A25134)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using TWIST1 Rabbit mAb (A25134) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TWIST1 Rabbit mAb (A25134)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using TWIST1 Rabbit mAb (A25134) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TWIST1 Rabbit mAb (A25134)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using TWIST1 Rabbit mAb (A25134) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TWIST1 Rabbit mAb (A25134)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using TWIST1 Rabbit mAb (A25134) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TWIST1 Rabbit mAb (A25134)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using TWIST1 Rabbit mAb (A25134) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TWIST1 Rabbit mAb (A25134)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using TWIST1 Rabbit mAb (A25134) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - TWIST1 Rabbit mAb (A25134)

Confocal imaging of SH-SY5Y cells using TWIST1 Rabbit mAb (A25134, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - TWIST1 Rabbit mAb (A25134)

Confocal imaging of paraffin-embedded Mouse brain tissue using TWIST1 Rabbit mAb (A25134, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Overview

Product nameTWIST1 Rabbit mAb
Catalog No.A25134
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60803
This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis, as well as metastatic recurrence. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-150 of human TWIST1(NP_000465.1).
SequenceAGPGGAAGGGVGGGDEPGSPAQGKRGKKSAGCGGGGGAGGGGGSSSGGGSPQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLK
Gene ID
Swiss Prot
SynonymsTWIST1; ACS3; BPES2; BPES3; CRS; CRS1; CSO; SCS; TWIST; bHLHa38; twist-related protein 1
Calculated MW21kDa
Observed MW26kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:1000 - 1:5000
  • IF/ICC 1:100 - 1:500
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSH-SY5Y
Cellular locationNucleus.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - TWIST1 Rabbit mAb (A25134)}

Western blot - TWIST1 Rabbit mAb (A25134)

Western blot analysis of various lysates using TWIST1 Rabbit mAb (A25134) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): K-562.
Exposure time: 90s.
ABclonal:Immunohistochemistry - TWIST1 Rabbit mAb (A25134)}

Immunohistochemistry - TWIST1 Rabbit mAb (A25134)

Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using TWIST1 Rabbit mAb (A25134) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TWIST1 Rabbit mAb (A25134)}

Immunohistochemistry - TWIST1 Rabbit mAb (A25134)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using TWIST1 Rabbit mAb (A25134) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TWIST1 Rabbit mAb (A25134)}

Immunohistochemistry - TWIST1 Rabbit mAb (A25134)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using TWIST1 Rabbit mAb (A25134) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TWIST1 Rabbit mAb (A25134)}

Immunohistochemistry - TWIST1 Rabbit mAb (A25134)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using TWIST1 Rabbit mAb (A25134) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TWIST1 Rabbit mAb (A25134)}

Immunohistochemistry - TWIST1 Rabbit mAb (A25134)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using TWIST1 Rabbit mAb (A25134) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TWIST1 Rabbit mAb (A25134)}

Immunohistochemistry - TWIST1 Rabbit mAb (A25134)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using TWIST1 Rabbit mAb (A25134) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TWIST1 Rabbit mAb (A25134)}

Immunohistochemistry - TWIST1 Rabbit mAb (A25134)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using TWIST1 Rabbit mAb (A25134) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - TWIST1 Rabbit mAb (A25134)}

Immunofluorescence - TWIST1 Rabbit mAb (A25134)

Confocal imaging of SH-SY5Y cells using TWIST1 Rabbit mAb (A25134, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - TWIST1 Rabbit mAb (A25134)}

Immunofluorescence - TWIST1 Rabbit mAb (A25134)

Confocal imaging of paraffin-embedded Mouse brain tissue using TWIST1 Rabbit mAb (A25134, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)