제품 > 항체 > 단일클론항체(mAb)

TrkB Rabbit mAb (A21227)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TrkB Rabbit mAb (A21227)

Western blot analysis of various lysates using TrkB Rabbit mAb (A21227) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - TrkB Rabbit mAb (A21227)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using TrkB Rabbit mAb (A21227) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TrkB Rabbit mAb (A21227)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using TrkB Rabbit mAb (A21227) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TrkB Rabbit mAb (A21227)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using TrkB Rabbit mAb (A21227) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TrkB Rabbit mAb (A21227)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using TrkB Rabbit mAb (A21227) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TrkB Rabbit mAb (A21227)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using TrkB Rabbit mAb (A21227) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TrkB Rabbit mAb (A21227)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using TrkB Rabbit mAb (A21227) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameTrkB Rabbit mAb
Catalog No.A21227
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC53483
This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 32-430 of human TrkB (NP_001018074.1).
SequenceCPTSCKCSASRIWCSDPSPGIVAFPRLEPNSVDPENITEIFIANQKRLEIINEDDVEAYVGLRNLTIVDSGLKFVAHKAFLKNSNLQHINFTRNKLTSLSRKHFRHLDLSELILVGNPFTCSCDIMWIKTLQEAKSSPDTQDLYCLNESSKNIPLANLQIPNCGLPSANLAAPNLTVEEGKSITLSCSVAGDPVPNMYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTVKGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYTLIAKNEYGKDEKQISAHFMGWPGIDDGANPNYPDVIYEDYGTAANDIGDTTNRSNEIPSTDVTDKTGREH
Gene ID
Swiss Prot
SynonymsOBHD; TRKB; DEE58; trk-B; EIEE58; GP145-TrkB; TrkB
Calculated MW92kDa
Observed MW90kDa/140kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:20000
  • IHC-P 1:500 - 1:5000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse brain, Rat brain
Cellular locationCell membrane, Endosome membrane, Single-pass type I membrane protein.
Customer validation

IF(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - TrkB Rabbit mAb (A21227)}

Western blot - TrkB Rabbit mAb (A21227)

Western blot analysis of various lysates using TrkB Rabbit mAb (A21227) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - TrkB Rabbit mAb (A21227)}

Immunohistochemistry - TrkB Rabbit mAb (A21227)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using TrkB Rabbit mAb (A21227) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TrkB Rabbit mAb (A21227)}

Immunohistochemistry - TrkB Rabbit mAb (A21227)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using TrkB Rabbit mAb (A21227) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TrkB Rabbit mAb (A21227)}

Immunohistochemistry - TrkB Rabbit mAb (A21227)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using TrkB Rabbit mAb (A21227) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TrkB Rabbit mAb (A21227)}

Immunohistochemistry - TrkB Rabbit mAb (A21227)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using TrkB Rabbit mAb (A21227) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TrkB Rabbit mAb (A21227)}

Immunohistochemistry - TrkB Rabbit mAb (A21227)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using TrkB Rabbit mAb (A21227) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TrkB Rabbit mAb (A21227)}

Immunohistochemistry - TrkB Rabbit mAb (A21227)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using TrkB Rabbit mAb (A21227) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A21227? Please let us know so that we can cite the reference in this datasheet.

항체 (4)

재조합 단백질 (1)

Secondary Antibodies (26)