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UBE2B Rabbit mAb (A0503)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UBE2B Rabbit mAb (A0503)

Western blot analysis of lysates from Rat testis, using UBE2B Rabbit mAb (A0503) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - UBE2B Rabbit mAb (A0503)

Western blot analysis of various lysates using UBE2B Rabbit mAb (A0503) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - UBE2B Rabbit mAb (A0503)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using UBE2B Rabbit mAb (A0503) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - UBE2B Rabbit mAb (A0503)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using UBE2B Rabbit mAb (A0503) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - UBE2B Rabbit mAb (A0503)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using UBE2B Rabbit mAb (A0503) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - UBE2B Rabbit mAb (A0503)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using UBE2B Rabbit mAb (A0503) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - UBE2B Rabbit mAb (A0503)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using UBE2B Rabbit mAb (A0503) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - UBE2B Rabbit mAb (A0503)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using UBE2B Rabbit mAb (A0503) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameUBE2B Rabbit mAb
Catalog No.A0503
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2512
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 53-152 of human UBE2B (P63146).
SequenceFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS
Gene ID
Swiss Prot
SynonymsHR6B; UBC2; HHR6B; RAD6B; E2-17kDa; UBE2B
Calculated MW17kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:500 - 1:5000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, 293T, NIH/3T3, K-562, Mouse testis, Mouse heart, Rat testis
Cellular locationCell membrane, Nucleus.

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - UBE2B Rabbit mAb (A0503)}

Western blot - UBE2B Rabbit mAb (A0503)

Western blot analysis of lysates from Rat testis, using UBE2B Rabbit mAb (A0503) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - UBE2B Rabbit mAb (A0503)}

Western blot - UBE2B Rabbit mAb (A0503)

Western blot analysis of various lysates using UBE2B Rabbit mAb (A0503) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - UBE2B Rabbit mAb (A0503)}

Immunohistochemistry - UBE2B Rabbit mAb (A0503)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using UBE2B Rabbit mAb (A0503) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - UBE2B Rabbit mAb (A0503)}

Immunohistochemistry - UBE2B Rabbit mAb (A0503)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using UBE2B Rabbit mAb (A0503) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - UBE2B Rabbit mAb (A0503)}

Immunohistochemistry - UBE2B Rabbit mAb (A0503)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using UBE2B Rabbit mAb (A0503) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - UBE2B Rabbit mAb (A0503)}

Immunohistochemistry - UBE2B Rabbit mAb (A0503)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using UBE2B Rabbit mAb (A0503) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - UBE2B Rabbit mAb (A0503)}

Immunohistochemistry - UBE2B Rabbit mAb (A0503)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using UBE2B Rabbit mAb (A0503) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - UBE2B Rabbit mAb (A0503)}

Immunohistochemistry - UBE2B Rabbit mAb (A0503)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using UBE2B Rabbit mAb (A0503) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)