제품 > 항체 > 다중클론항체(pAb)

UGCG Rabbit pAb (A6317)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UGCG Rabbit pAb (A6317)

Western blot analysis of lysates from HeLa cells using UGCG Rabbit pAb(A6317) at 1:2000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime:60s.

Overview

Product nameUGCG Rabbit pAb
Catalog No.A6317
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an enzyme that catalyzes the first glycosylation step in the biosynthesis of glycosphingolipids, which are membrane components containing lipid and sugar moieties. The product of this reaction is glucosylceramide, which is the core structure of many glycosphingolipids.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human UGCG (NP_003349.1).
SequenceMALLDLALEGMAVFGFVLFLVLWLMHFMAIIYTRLHLNKKATDKQPYSKLPGVSLLKPLKGVDPNLINNLETFFELDYPKYEVLLCVQDHDDPAIDVCKK
Gene ID
Swiss Prot
SynonymsGCS; GLCT1; UGCG
Calculated MW45kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa
Cellular locationGolgi apparatus membrane, Multi-pass membrane protein
Customer validation

IHC(Mesocricetus auratus)

Documents

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    ABclonal:Western blot - UGCG Rabbit pAb (A6317)}

    Western blot - UGCG Rabbit pAb (A6317)

    Western blot analysis of lysates from HeLa cells using UGCG Rabbit pAb(A6317) at 1:2000 dilution.
    Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection:ECL Basic Kit (RM00020).
    Exposuretime:60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A6317? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)