제품 > 항체 > 다중클론항체(pAb)

UGT1A6 Rabbit pAb (A17166)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UGT1A6 Rabbit pAb (A17166)

Western blot analysis of extracts of various cell lines, using UGT1A6 Rabbit pAb (A17166) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - UGT1A6 Rabbit pAb (A17166)

Immunofluorescence analysis of rat liver using UGT1A6 antibody (A17166) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - UGT1A6 Rabbit pAb (A17166)

Immunofluorescence analysis of mouse liver using UGT1A6 antibody (A17166) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameUGT1A6 Rabbit pAb
Catalog No.A17166
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The enzyme encoded by this gene is active on phenolic and planar compounds. Alternative splicing in the unique 5' end of this gene results in two transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 65-270 of human UGT1A6 (NP_001063.2).
SequenceLLLKESKYYTRKIYPVPYDQEELKNRYQSFGNNHFAERSFLTAPQTEYRNNMIVIGLYFINCQSLLQDRDTLNFFKESKFDALFTDPALPCGVILAEYLGLPSVYLFRGFPCSLEHTFSRSPDPVSYIPRCYTKFSDHMTFSQRVANFLVNLLEPYLFYCLFSKYEELASAVLKRDVDIITLYQKVSVWLLRYDFVLEYPRPVMPN
Gene ID
Swiss Prot
SynonymsGNT1; UGT1; HLUGP; UDPGT; UGT1A; UGT1C; UGT1E; UGT1F; HLUGP1; UGT-1A; UGT-1C; UGT-1E; UGT-1F; UGT1.1; UGT1.3; UGT1.5; UGT1.6; UGT1A1; UGT1A3; UGT1A5; UGT1-01; UGT1-03; UGT1-05; UGT1-06; UGT1A6S; hUG-BR1; UDPGT 1-6
Calculated MW61kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesA-431, HT-29, Mouse liver, Mouse kidney
Cellular locationEndoplasmic reticulum membrane, Microsome, Single-pass membrane protein

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ABclonal:Western blot - UGT1A6 Rabbit pAb (A17166)}

Western blot - UGT1A6 Rabbit pAb (A17166)

Western blot analysis of extracts of various cell lines, using UGT1A6 Rabbit pAb (A17166) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - UGT1A6 Rabbit pAb (A17166)}

Immunofluorescence - UGT1A6 Rabbit pAb (A17166)

Immunofluorescence analysis of rat liver using UGT1A6 antibody (A17166) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - UGT1A6 Rabbit pAb (A17166)}

Immunofluorescence - UGT1A6 Rabbit pAb (A17166)

Immunofluorescence analysis of mouse liver using UGT1A6 antibody (A17166) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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