제품 > 항체 > 다중클론항체(pAb)

UPK2 Rabbit pAb (A10813)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from B-cell cells, using UPK2 Rabbit pAb (A10813) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 40s.

Overview

Product name	UPK2 Rabbit pAb
Catalog No.	A10813
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes one of the proteins of the highly conserved urothelium-specific integral membrane proteins of the asymmetric unit membrane which forms urothelium apical plaques in mammals. The asymmetric unit membrane is believed to strengthen the urothelium by preventing cell rupture during bladder distention. The encoded protein is expressed in the peripheral blood of bladder cancer patients with transitional cell carcinomas.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 85-155 of human UPK2 (NP_006751.1).
Sequence	ELVSVVDSGAGFTVTRLSAYQVTNLVPGTKFYISYLVKKGTATESSREIPMSTLPRRNMESIGLGMARTGG
Gene ID
Swiss Prot
Synonyms	UP2; UPII; UPK2
Calculated MW	19kDa
Observed MW	19kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	B-cell
Cellular location	Cell membrane, Single-pass type I membrane protein
Customer validation	WB(Homo sapiens)