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USP18 Rabbit pAb (A16739)

Publications (8) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - USP18 Rabbit pAb (A16739)

Western blot analysis of various lysates using USP18 Rabbit pAb (A16739) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - USP18 Rabbit pAb (A16739)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using USP18 Rabbit pAb (A16739) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - USP18 Rabbit pAb (A16739)

Immunohistochemistry analysis of paraffin-embedded Human colon using USP18 Rabbit pAb (A16739) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - USP18 Rabbit pAb (A16739)

Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using USP18 Rabbit pAb (A16739) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - USP18 Rabbit pAb (A16739)

Immunohistochemistry analysis of paraffin-embedded Rat brain using USP18 Rabbit pAb (A16739) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - USP18 Rabbit pAb (A16739)

Immunofluorescence analysis of MCF7 cells using USP18 Rabbit pAb (A16739) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameUSP18 Rabbit pAb
Catalog No.A16739
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the ubiquitin-specific proteases (UBP) family of enzymes that cleave ubiquitin from ubiquitinated protein substrates. It is highly expressed in liver and thymus, and is localized to the nucleus. This protein efficiently cleaves only ISG15 (a ubiquitin-like protein) fusions, and deletion of this gene in mice results in a massive increase of ISG15 conjugates in tissues, indicating that this protein is a major ISG15-specific protease. Mice lacking this gene are also hypersensitive to interferon, suggesting a function of this protein in downregulating interferon responses, independent of its isopeptidase activity towards ISG15.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human USP18 (NP_059110.2).
SequenceMSKAFGLLRQICQSILAESSQSPADLEEKKEEDSNMKREQPRERPRAWDYPHGLVGLHNIGQTCCLNSLIQVFVMNVDFTRILKRITVPRGADEQRRSVP
Gene ID
Swiss Prot
SynonymsISG43; UBP43; PTORCH2; USP18
Calculated MW43kDa
Observed MW34kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHep G2, Mouse liver
Cellular locationcytosol, nucleus.
Customer validation

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

IHC(Mus musculus, Homo sapiens)

IF(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - USP18 Rabbit pAb (A16739)}

    Western blot - USP18 Rabbit pAb (A16739)

    Western blot analysis of various lysates using USP18 Rabbit pAb (A16739) at 1:3000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates / proteins: 25 μg per lane.
    Blocking buffer: 3 % nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunohistochemistry - USP18 Rabbit pAb (A16739)}

    Immunohistochemistry - USP18 Rabbit pAb (A16739)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using USP18 Rabbit pAb (A16739) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - USP18 Rabbit pAb (A16739)}

    Immunohistochemistry - USP18 Rabbit pAb (A16739)

    Immunohistochemistry analysis of paraffin-embedded Human colon using USP18 Rabbit pAb (A16739) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - USP18 Rabbit pAb (A16739)}

    Immunohistochemistry - USP18 Rabbit pAb (A16739)

    Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using USP18 Rabbit pAb (A16739) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - USP18 Rabbit pAb (A16739)}

    Immunohistochemistry - USP18 Rabbit pAb (A16739)

    Immunohistochemistry analysis of paraffin-embedded Rat brain using USP18 Rabbit pAb (A16739) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - USP18 Rabbit pAb (A16739)}

    Immunofluorescence - USP18 Rabbit pAb (A16739)

    Immunofluorescence analysis of MCF7 cells using USP18 Rabbit pAb (A16739) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A16739? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (26)