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USP39 Rabbit pAb (A9582)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - USP39 Rabbit pAb (A9582)

Western blot analysis of various lysates using USP39 Rabbit pAb (A9582) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product nameUSP39 Rabbit pAb
Catalog No.A9582
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable thiol-dependent deubiquitinase and zinc ion binding activity. Involved in spliceosomal complex assembly. Located in nucleoplasm. Part of U4/U6 x U5 tri-snRNP complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 336-565 of human USP39 (NP_006581.2).
SequenceGTKKKKKTIVTDVFQGSMRIFTKKLPHPDLPAEEKEQLLHNDEYQETMVESTFMYLTLDLPTAPLYKDEKEQLIIPQVPLFNILAKFNGITEKEYKTYKENFLKRFQLTKLPPYLIFCIKRFTKNNFFVEKNPTIVNFPITNVDLREYLSEEVQAVHKNTTYDLIANIVHDGKPSEGSYRIHVLHHGTGKWYELQDLQVTDILPQMITLSEAYIQIWKRRDNDETNQQGA
Gene ID
Swiss Prot
Synonyms65K; SAD1; CGI-21; HSPC332; SNRNP65; USP39
Calculated MW65kDa
Observed MW65kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHepG2, HeLa, A375, HT-29, B-cell, Mouse eye, Mouse spleen, Rat testis
Cellular locationNucleus
Customer validation

WB(Mus musculus)

Documents

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    ABclonal:Western blot - USP39 Rabbit pAb (A9582)}

    Western blot - USP39 Rabbit pAb (A9582)

    Western blot analysis of various lysates using USP39 Rabbit pAb (A9582) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A9582? Please let us know so that we can cite the reference in this datasheet.

    항체 (2)

    Secondary Antibodies (26)