제품 > 항체 > 다중클론항체(pAb)

VAMP8 Rabbit pAb (A13915)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - VAMP8 Rabbit pAb (A13915)

Western blot analysis of various lysates using VAMP8 Rabbit pAb (A13915) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameVAMP8 Rabbit pAb
Catalog No.A13915
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an integral membrane protein that belongs to the synaptobrevin/vesicle-associated membrane protein subfamily of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). The encoded protein is involved in the fusion of synaptic vesicles with the presynaptic membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-75 of human VAMP8 (NP_003752.2).
SequenceMEEASEGGGNDRVRNLQSEVEGVKNIMTQNVERILARGENLEHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVK
Gene ID
Swiss Prot
SynonymsEDB; VAMP-8; VAMP8
Calculated MW11kDa
Observed MW11kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, NIH/3T3, PC-12, Mouse kidney, Mouse liver, Rat lung
Cellular locationCell membrane, Early endosome membrane, Late endosome membrane, Lysosome membrane, Single-pass type IV membrane protein
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - VAMP8 Rabbit pAb (A13915)}

Western blot - VAMP8 Rabbit pAb (A13915)

Western blot analysis of various lysates using VAMP8 Rabbit pAb (A13915) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A13915? Please let us know so that we can cite the reference in this datasheet.