제품 > 항체 > 다중클론항체(pAb)

VARS Rabbit pAb (A4182)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - VARS Rabbit pAb (A4182)

Western blot analysis of various lysates using VARS Rabbit pAb (A4182) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - VARS Rabbit pAb (A4182)

Immunohistochemistry analysis of paraffin-embedded rat brain using VARS Rabbit pAb (A4182) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - VARS Rabbit pAb (A4182)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using VARS Rabbit pAb (A4182) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - VARS Rabbit pAb (A4182)

Immunohistochemistry analysis of paraffin-embedded mouse brain using VARS Rabbit pAb (A4182) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - VARS Rabbit pAb (A4182)

Immunofluorescence analysis of L929 cells using VARS Rabbit pAb (A4182) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameVARS Rabbit pAb
Catalog No.A4182
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human VARS (NP_006286.1).
SequenceMSTLYVSPHPDAFPSLRALIAARYGEAGEGPGWGGAHPRICLQPPPTSRTPFPPPRLPALEQGPGGLWVWGATAVAQLLWPAGLGGPGGSRAAVLVQQWVSYADTELIPAACGATLPALGLRSSAQDPQAVLGALGRALSPLEEWLRLHTYLAGEAPTLADLAAVTALLLPFRYVLDPPARRIWNNVTRWFVTCVRQPEFRAVLGEVVLYSGARPLSHQPGPEAPALPKTAAQLKKEAKKREKLEKFQQKQKIQQQQPPPGEKKPKPEKREKRDPGVITY
Gene ID
Swiss Prot
SynonymsG7A; VARS; VARS2; NDMSCA
Calculated MW140kDa
Observed MW140kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, HL-60, K-562, SW620, Mouse brain, Mouse heart, Mouse liver, Rat liver
Cellular locationcytosol
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - VARS Rabbit pAb (A4182)}

    Western blot - VARS Rabbit pAb (A4182)

    Western blot analysis of various lysates using VARS Rabbit pAb (A4182) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - VARS Rabbit pAb (A4182)}

    Immunohistochemistry - VARS Rabbit pAb (A4182)

    Immunohistochemistry analysis of paraffin-embedded rat brain using VARS Rabbit pAb (A4182) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - VARS Rabbit pAb (A4182)}

    Immunohistochemistry - VARS Rabbit pAb (A4182)

    Immunohistochemistry analysis of paraffin-embedded human breast cancer using VARS Rabbit pAb (A4182) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - VARS Rabbit pAb (A4182)}

    Immunohistochemistry - VARS Rabbit pAb (A4182)

    Immunohistochemistry analysis of paraffin-embedded mouse brain using VARS Rabbit pAb (A4182) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - VARS Rabbit pAb (A4182)}

    Immunofluorescence - VARS Rabbit pAb (A4182)

    Immunofluorescence analysis of L929 cells using VARS Rabbit pAb (A4182) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A4182? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)