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VEGFB Rabbit pAb (A2132)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - VEGFB Rabbit pAb (A2132)

Western blot analysis of extracts of Rat heart, using VEGFB Rabbit pAb (A2132) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunofluorescence - VEGFB Rabbit pAb (A2132)

Immunofluorescence analysis of C6 cells using VEGFB Rabbit pAb (A2132) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - VEGFB Rabbit pAb (A2132)

Immunofluorescence analysis of NIH-3T3 cells using VEGFB Rabbit pAb (A2132) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - VEGFB Rabbit pAb (A2132)

Immunofluorescence analysis of U-2 OS cells using VEGFB Rabbit pAb (A2132) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameVEGFB Rabbit pAb
Catalog No.A2132
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the PDGF (platelet-derived growth factor)/VEGF (vascular endothelial growth factor) family. The VEGF family members regulate the formation of blood vessels and are involved in endothelial cell physiology. This member is a ligand for VEGFR-1 (vascular endothelial growth factor receptor 1) and NRP-1 (neuropilin-1). Studies in mice showed that this gene was co-expressed with nuclear-encoded mitochondrial genes and the encoded protein specifically controlled endothelial uptake of fatty acids. Alternatively spliced transcript variants encoding distinct isoforms have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 30-121 of human VEGFB (NP_003368.1).
SequenceGHQRKVVSWIDVYTRATCQPREVVVPLTVELMGTVAKQLVPSCVTVQRCGGCCPDDGLECVPTGQHQVRMQILMIRYPSSQLGEMSLEEHSQ
Gene ID
Swiss Prot
SynonymsVRF; VEGFL
Calculated MW22kDa
Observed MW32kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRat heart
Cellular locationSecreted

Documents

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ABclonal:Western blot - VEGFB Rabbit pAb (A2132)}

Western blot - VEGFB Rabbit pAb (A2132)

Western blot analysis of extracts of Rat heart, using VEGFB Rabbit pAb (A2132) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunofluorescence - VEGFB Rabbit pAb (A2132)}

Immunofluorescence - VEGFB Rabbit pAb (A2132)

Immunofluorescence analysis of C6 cells using VEGFB Rabbit pAb (A2132) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - VEGFB Rabbit pAb (A2132)}

Immunofluorescence - VEGFB Rabbit pAb (A2132)

Immunofluorescence analysis of NIH-3T3 cells using VEGFB Rabbit pAb (A2132) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - VEGFB Rabbit pAb (A2132)}

Immunofluorescence - VEGFB Rabbit pAb (A2132)

Immunofluorescence analysis of U-2 OS cells using VEGFB Rabbit pAb (A2132) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)