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VPS18 Rabbit mAb (A17563)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - VPS18 Rabbit mAb (A17563)

Western blot analysis of extracts of various cell lines, using VPS18 Rabbit mAb (A17563) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - VPS18 Rabbit mAb (A17563)

Western blot analysis of extracts of Rat brain, using VPS18 Rabbit mAb (A17563) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product nameVPS18 Rabbit mAb
Catalog No.A17563
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2156
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human VPS18 (Q9P253).
SequenceMASILDEYENSLSRSAVLQPGCPSVGIPHSGYVNAQLEKEVPIFTKQRIDFTPSERITSLVVSSNQLCMSLGKDTLLRIDLGKANEPNHVELGRKDDAKV
Gene ID
Swiss Prot
SynonymsPEP3
Calculated MW110kDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, Mouse lung, Mouse brain, Rat brain
Cellular locationAutophagosome, Clathrin-coated vesicle, CORVET complex, Early endosome, endosome, Glutamatergic synapse, Late endosome, Lysosome, Presynapse
Customer validation

IP(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - VPS18 Rabbit mAb (A17563)}

    Western blot - VPS18 Rabbit mAb (A17563)

    Western blot analysis of extracts of various cell lines, using VPS18 Rabbit mAb (A17563) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Western blot - VPS18 Rabbit mAb (A17563)}

    Western blot - VPS18 Rabbit mAb (A17563)

    Western blot analysis of extracts of Rat brain, using VPS18 Rabbit mAb (A17563) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A17563? Please let us know so that we can cite the reference in this datasheet.

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