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VPS35 Rabbit pAb (A7117)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - VPS35 Rabbit pAb (A7117)

Western blot analysis of extracts of various cell lines, using VPS35 antibody (A7117) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.

Overview

Product nameVPS35 Rabbit pAb
Catalog No.A7117
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human VPS35 (NP_060676.2).
SequenceMPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQV
Gene ID
Swiss Prot
SynonymsMEM3; PARK17
Calculated MW92kDa
Observed MW92kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, K-562, Mouse liver, Mouse thymus
Cellular locationCytoplasm, Early endosome, Endosome, Late endosome, Membrane, Peripheral membrane protein

Documents

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    ABclonal:Western blot - VPS35 Rabbit pAb (A7117)}

    Western blot - VPS35 Rabbit pAb (A7117)

    Western blot analysis of extracts of various cell lines, using VPS35 antibody (A7117) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)