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WBP11 Rabbit pAb (A16191)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - WBP11 Rabbit pAb (A16191)

Western blot analysis of various lysates using WBP11 Rabbit pAb (A16191) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - WBP11 Rabbit pAb (A16191)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using WBP11 Rabbit pAb (A16191) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - WBP11 Rabbit pAb (A16191)

Immunohistochemistry analysis of paraffin-embedded mouse lung using WBP11 Rabbit pAb (A16191) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - WBP11 Rabbit pAb (A16191)

Immunohistochemistry analysis of paraffin-embedded rat liver using WBP11 Rabbit pAb (A16191) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - WBP11 Rabbit pAb (A16191)

Immunofluorescence analysis of NIH/3T3 cells using WBP11 Rabbit pAb (A16191) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - WBP11 Rabbit pAb (A16191)

Immunofluorescence analysis of U2OS cells using WBP11 Rabbit pAb (A16191) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameWBP11 Rabbit pAb
Catalog No.A16191
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a nuclear protein, which colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. This protein has 95% amino acid sequence identity to the mouse Wbp11 protein. It contains two proline-rich regions that bind to the WW domain of Npw38, a nuclear protein, and thus this protein is also called Npw38-binding protein NpwBP. The Npw38-NpwBP complex may function as a component of an mRNA factory in the nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 540-641 of human WBP11 (NP_057396.1).
SequenceLIQRPKADDTSAATIEKKATATISAKPQITNPKAEITRFVPTALRVRRENKGATAAPQRKSEDDSAVPLAKAAPKSGPSVPVSVQTKDDVYEAFMKEMEGLL
Gene ID
Swiss Prot
SynonymsBUG13; FAP90; NPWBP; SIPP1; VCTRL; CFAP90; VCTERL; WBP-11; PPP1R165; WBP11
Calculated MW70kDa
Observed MW115kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRD, Mouse testis
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mus musculus, Homo sapiens)

Documents

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    ABclonal:Western blot - WBP11 Rabbit pAb (A16191)}

    Western blot - WBP11 Rabbit pAb (A16191)

    Western blot analysis of various lysates using WBP11 Rabbit pAb (A16191) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - WBP11 Rabbit pAb (A16191)}

    Immunohistochemistry - WBP11 Rabbit pAb (A16191)

    Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using WBP11 Rabbit pAb (A16191) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - WBP11 Rabbit pAb (A16191)}

    Immunohistochemistry - WBP11 Rabbit pAb (A16191)

    Immunohistochemistry analysis of paraffin-embedded mouse lung using WBP11 Rabbit pAb (A16191) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - WBP11 Rabbit pAb (A16191)}

    Immunohistochemistry - WBP11 Rabbit pAb (A16191)

    Immunohistochemistry analysis of paraffin-embedded rat liver using WBP11 Rabbit pAb (A16191) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - WBP11 Rabbit pAb (A16191)}

    Immunofluorescence - WBP11 Rabbit pAb (A16191)

    Immunofluorescence analysis of NIH/3T3 cells using WBP11 Rabbit pAb (A16191) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - WBP11 Rabbit pAb (A16191)}

    Immunofluorescence - WBP11 Rabbit pAb (A16191)

    Immunofluorescence analysis of U2OS cells using WBP11 Rabbit pAb (A16191) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A16191? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)