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YEATS4 Rabbit pAb (A6318)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - YEATS4 Rabbit pAb (A6318)

Western blot analysis of lysates from 293T cells, using YEATS4 Rabbit pAb (A6318) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - YEATS4 Rabbit pAb (A6318)

Immunohistochemistry analysis of paraffin-embedded Rat lung using YEATS4 Rabbit pAb (A6318) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - YEATS4 Rabbit pAb (A6318)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using YEATS4 Rabbit pAb (A6318) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - YEATS4 Rabbit pAb (A6318)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using YEATS4 Rabbit pAb (A6318) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - YEATS4 Rabbit pAb (A6318)

Immunofluorescence analysis of A-549 cells using YEATS4 Rabbit pAb (A6318) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - YEATS4 Rabbit pAb (A6318)

Immunofluorescence analysis of HeLa cells using YEATS4 Rabbit pAb (A6318) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - YEATS4 Rabbit pAb (A6318)

Immunofluorescence analysis of U2OS cells using YEATS4 Rabbit pAb (A6318) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameYEATS4 Rabbit pAb
Catalog No.A6318
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is found in the nucleoli. It has high sequence homology to human MLLT1, and yeast and human MLLT3 proteins. Both MLLT1 and MLLT3 proteins belong to a class of transcription factors, indicating that the encoded protein might also represent a transcription factor. This protein is thought to be required for RNA transcription. This gene has been shown to be amplified in tumors. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-227 of human YEATS4 (NP_006521.1).
SequenceMFKRMAEFGPDSGGRVKGVTIVKPIVYGNVARYFGKKREEDGHTHQWTVYVKPYRNEDMSAYVKKIQFKLHESYGNPLRVVTKPPYEITETGWGEFEIIIKIFFIDPNERPVTLYHLLKLFQSDTNAMLGKKTVVSEFYDEMIFQDPTAMMQQLLTTSRQLTLGAYKHETEFAELEVKTREKLEAAKKKTSFEIAELKERLKASRETINCLKNEIRKLEEDDQAKDI
Gene ID
Swiss Prot
SynonymsYAF9; GAS41; NUBI-1; 4930573H17Rik; B230215M10Rik; YEATS4
Calculated MW26kDa
Observed MW27kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T
Cellular locationNucleus
Customer validation

IP(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - YEATS4 Rabbit pAb (A6318)}

Western blot - YEATS4 Rabbit pAb (A6318)

Western blot analysis of lysates from 293T cells, using YEATS4 Rabbit pAb (A6318) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - YEATS4 Rabbit pAb (A6318)}

Immunohistochemistry - YEATS4 Rabbit pAb (A6318)

Immunohistochemistry analysis of paraffin-embedded Rat lung using YEATS4 Rabbit pAb (A6318) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - YEATS4 Rabbit pAb (A6318)}

Immunohistochemistry - YEATS4 Rabbit pAb (A6318)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using YEATS4 Rabbit pAb (A6318) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - YEATS4 Rabbit pAb (A6318)}

Immunohistochemistry - YEATS4 Rabbit pAb (A6318)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using YEATS4 Rabbit pAb (A6318) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - YEATS4 Rabbit pAb (A6318)}

Immunofluorescence - YEATS4 Rabbit pAb (A6318)

Immunofluorescence analysis of A-549 cells using YEATS4 Rabbit pAb (A6318) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - YEATS4 Rabbit pAb (A6318)}

Immunofluorescence - YEATS4 Rabbit pAb (A6318)

Immunofluorescence analysis of HeLa cells using YEATS4 Rabbit pAb (A6318) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - YEATS4 Rabbit pAb (A6318)}

Immunofluorescence - YEATS4 Rabbit pAb (A6318)

Immunofluorescence analysis of U2OS cells using YEATS4 Rabbit pAb (A6318) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A6318? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)