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YTHDF2 Rabbit pAb (A24287)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - YTHDF2 Rabbit pAb (A24287)

Western blot analysis of lysates from HeLa cells, using YTHDF2 Rabbit pAb (A24287) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - YTHDF2 Rabbit pAb (A24287)

Immunohistochemistry analysis of paraffin-embedded Human colon using YTHDF2 Rabbit pAb (A24287) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - YTHDF2 Rabbit pAb (A24287)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using YTHDF2 Rabbit pAb (A24287) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - YTHDF2 Rabbit pAb (A24287)

Immunofluorescence analysis of HeLa cells using YTHDF2 Rabbit pAb (A24287) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - YTHDF2 Rabbit pAb (A24287)

Immunofluorescence analysis of U-2 OS cells using YTHDF2 Rabbit pAb (A24287) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameYTHDF2 Rabbit pAb
Catalog No.A24287
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the YTH (YT521-B homology) superfamily containing YTH domain. The YTH domain is typical for the eukaryotes and is particularly abundant in plants. The YTH domain is usually located in the middle of the protein sequence and may function in binding to RNA. In addition to a YTH domain, this protein has a proline rich region which may be involved in signal transduction. An Alu-rich domain has been identified in one of the introns of this gene, which is thought to be associated with human longevity. In addition, reciprocal translocations between this gene and the Runx1 (AML1) gene on chromosome 21 has been observed in patients with acute myeloid leukemia. This gene was initially mapped to chromosome 14, which was later turned out to be a pseudogene. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 143-243AA of human YTHDF2 (NP_001166599.1).
SequenceSTQSSGYSSNYAYAPSSLGGAMIDGQSAFANETLNKAPGMNTIDQGMAALKLGSTEVASNVPKVVGSAVGSGSITSNIVASNSLPPATIAPPKPASWADIA
Gene ID
Swiss Prot
SynonymsDF2; CAHL; HGRG8; NY-REN-2; YTHDF2
Calculated MW62kDa
Observed MW65kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa
Cellular location

Documents

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ABclonal:Western blot - YTHDF2 Rabbit pAb (A24287)}

Western blot - YTHDF2 Rabbit pAb (A24287)

Western blot analysis of lysates from HeLa cells, using YTHDF2 Rabbit pAb (A24287) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - YTHDF2 Rabbit pAb (A24287)}

Immunohistochemistry - YTHDF2 Rabbit pAb (A24287)

Immunohistochemistry analysis of paraffin-embedded Human colon using YTHDF2 Rabbit pAb (A24287) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - YTHDF2 Rabbit pAb (A24287)}

Immunohistochemistry - YTHDF2 Rabbit pAb (A24287)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using YTHDF2 Rabbit pAb (A24287) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - YTHDF2 Rabbit pAb (A24287)}

Immunofluorescence - YTHDF2 Rabbit pAb (A24287)

Immunofluorescence analysis of HeLa cells using YTHDF2 Rabbit pAb (A24287) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - YTHDF2 Rabbit pAb (A24287)}

Immunofluorescence - YTHDF2 Rabbit pAb (A24287)

Immunofluorescence analysis of U-2 OS cells using YTHDF2 Rabbit pAb (A24287) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)