제품 > 항체 > 다중클론항체(pAb)

ZNF263 Rabbit pAb (A16477)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ZNF263 Rabbit pAb (A16477)

Western blot analysis of various lysates, using ZNF263 Rabbit pAb (A16477) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - ZNF263 Rabbit pAb (A16477)

Western blot analysis of various lysates, using ZNF263 Rabbit pAb (A16477) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - ZNF263 Rabbit pAb (A16477)

Immunohistochemistry analysis of paraffin-embedded Human colon using ZNF263 Rabbit pAb (A16477) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameZNF263 Rabbit pAb
Catalog No.A16477
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables DNA-binding transcription repressor activity, RNA polymerase II-specific and transcription cis-regulatory region binding activity. Involved in negative regulation of transcription by RNA polymerase II and positive regulation of transcription by RNA polymerase II. Located in nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 120-240 of human ZNF263 (NP_005732.2).
SequenceQRELGRLRQQVTNHGRGTEVLLEEPLPLETARESPSFKLEPMETERSPGPRLQELLGPSPQRDPQAVKERALSAPWLSLFPPEGNMEDKEMTGPQLPESLEDVAMYISQEEWGHQDPSKRA
Gene ID
Swiss Prot
SynonymsFPM315; ZSCAN44; ZKSCAN12; ZNF263
Calculated MW77kDa
Observed MW77kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesU-87MG, 293F, Mouse brain, Mouse spleen, Rat brain
Cellular locationNucleus

Documents

Certificate of Compliance

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ABclonal:Western blot - ZNF263 Rabbit pAb (A16477)}

Western blot - ZNF263 Rabbit pAb (A16477)

Western blot analysis of various lysates, using ZNF263 Rabbit pAb (A16477) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - ZNF263 Rabbit pAb (A16477)}

Western blot - ZNF263 Rabbit pAb (A16477)

Western blot analysis of various lysates, using ZNF263 Rabbit pAb (A16477) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - ZNF263 Rabbit pAb (A16477)}

Immunohistochemistry - ZNF263 Rabbit pAb (A16477)

Immunohistochemistry analysis of paraffin-embedded Human colon using ZNF263 Rabbit pAb (A16477) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)