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ZNF471 Rabbit pAb (A16560)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - ZNF471 Rabbit pAb (A16560)

Western blot analysis of various lysates using ZNF471 Rabbit pAb (A16560) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameZNF471 Rabbit pAb
Catalog No.A16560
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Located in nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 80-210 of human ZNF471 (NP_065864.2).
SequenceTRSPFSDWESIYVTQELPLKQFMYDDACMEGITSYGLECSTFEENWKWEDLFEKQMGSHEMFSKKEIITHKETITKETEFKYTKFGKCIHLENIEESIYNHTSDKKSFSKNSMVIKHKKVYVGKKLFKCNE
Gene ID
Swiss Prot
SynonymsERP1; Z1971; Zfp78; ZNF471
Calculated MW73kDa
Observed MW90kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse pancreas, Mouse skeletal muscle, Mouse brain, Rat pancreas, Rat skeletal muscle, Rat brain
Cellular locationNucleus
ABclonal:Western blot - ZNF471 Rabbit pAb (A16560)}

Western blot - ZNF471 Rabbit pAb (A16560)

Western blot analysis of various lysates using ZNF471 Rabbit pAb (A16560) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)