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ZNF76 Rabbit pAb (A24491)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - ZNF76 Rabbit pAb (A24491)

Western blot analysis of Mouse testis, using ZNF76 Rabbit pAb (A24491) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameZNF76 Rabbit pAb
Catalog No.A24491
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables DNA-binding transcription activator activity, RNA polymerase II-specific and sequence-specific double-stranded DNA binding activity. Involved in positive regulation of transcription by RNA polymerase II. Predicted to be located in nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 463-570 of human ZNF76 (NP_003418.2).
SequenceQHGSTTLTIPSPDADLATSGTHTVTMVSADGTQTQPVTIITSGAVVAEDSSVASLRHQQVALLATANGTHIAVQLEEQQTLEEAINVATAAMQQGAVTLETTVSESGC
Gene ID
Swiss Prot
SynonymsZNF76; D6S229E; ZNF523; Zfp523; zinc finger protein 76
Calculated MW56kDa/61kDa
Observed MW62kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse testis
Cellular locationnucleus

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ABclonal:Western blot - ZNF76 Rabbit pAb (A24491)}

Western blot - ZNF76 Rabbit pAb (A24491)

Western blot analysis of Mouse testis, using ZNF76 Rabbit pAb (A24491) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)