제품 > 항체 > 다중클론항체(pAb)

ZSCAN1 Rabbit pAb (A16639)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - ZSCAN1 Rabbit pAb (A16639)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ZSCAN1 using ZSCAN1 Rabbit pAb (A16639) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - ZSCAN1 Rabbit pAb (A16639)

Western blot analysis of lysates from T-47D cells, using ZSCAN1 Rabbit pAb (A16639) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product nameZSCAN1 Rabbit pAb
Catalog No.A16639
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be located in nucleus. Predicted to be part of chromatin.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300-400 of human ZSCAN1 (NP_872378.3).
SequenceVFTWVTHFIEHQKTHREEGPFPCPECGKVFLHNSVLTEHGKIHLLEPPRKKAPRSKGPRESVPPRDGAQGPVAPRSPKRPFQCSVCGKAFPWMVHLIDHQK
Gene ID
Swiss Prot
SynonymsMZF-1; ZNF915; ZSCAN1
Calculated MW45kDa
Observed MW45kDa/62kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T transfected with ZSCAN1, T-47D
Cellular locationnucleus.

Documents

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ABclonal:Western blot - ZSCAN1 Rabbit pAb (A16639)}

Western blot - ZSCAN1 Rabbit pAb (A16639)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ZSCAN1 using ZSCAN1 Rabbit pAb (A16639) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - ZSCAN1 Rabbit pAb (A16639)}

Western blot - ZSCAN1 Rabbit pAb (A16639)

Western blot analysis of lysates from T-47D cells, using ZSCAN1 Rabbit pAb (A16639) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)