Biotinylated Recombinant SARS-COV-2 Spike S1+S2 ECD (S-ECD) Trimer Protein (RP02331)

Product name	Biotinylated Recombinant SARS-COV-2 Spike S1+S2 ECD (S-ECD) Trimer Protein
Catalog No.	RP02331
Description	Biotinylated Recombinant SARS-COV-2 Spike S Trimer Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Val16-Glu1188) of SARS-COV-2 Spike S Trimer fused with a His tag and Avi at the C-terminal.
Bio-Activity	Immobilized Biotinylated SARS-COV-2 Spike S, His Tag at 0.5 μg/mL (100 μL/Well).Dose response curve for Human ACE2, hFc Tag with the EC₅₀ of 36.5 ng/mL determined by ELISA.
Purity	> 95% by Tris-Bis PAGE
Endotoxin	< 1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Species	SARS-COV-2
Calculated MW	136.6 kDa

Synonym	S protein; Spike glycoprotein; S glycoprotein; COVID-19
Expression Host	HEK293 cells
Tag	C-His&Avi
Swiss-Prot
Gene ID

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.