Overview
| Product name | Recombinant SARS-CoV-2 Spike RBD(B.1.1.529/Omicron) Protein |
|---|
| Catalog No. | RP02107 |
|---|
| Description | Recombinant SARS-COV-2 Spike RBD(B.1.1.529/Omicron) is produced by Expi293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541(G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H)) of SARS-COV-2 Spike RBD(B.1.1.529/Omicron) (Accession #QHO60594.1) fused with His tag at the C-terminus. |
|---|
| Bio-Activity | Immobilized SARS-COV-2 Spike RBD (B.1.1.529/Omicron) His Tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 7.5 ng/mL determined by ELISA.
|
|---|
| Purity | >95% by SDS-PAGE;> 95% by HPLC |
|---|
| Endotoxin | < 1 EU/μg of the protein by LAL method. |
|---|
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
|---|
| Species | SARS-CoV-2 |
|---|
| Calculated MW | 26.2 kDa |
|---|
Background
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Product information
| Synonym | S1-RBD protein; NCP-CoV RBD Protein; novel coronavirus RBD Protein; 2019-nCoV RBD Protein; S glycoprotein Subunit1 RBD Protein |
|---|
| Expression Host | HEK293 cells |
|---|
| Tag | C-His |
|---|
| Swiss-Prot | |
|---|
| Gene ID | |
|---|
Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|
| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
|---|
* For research use only. Not for therapeutic or diagnostic purposes.
