Overview
| Product name | Recombinant SARS-CoV-2 Spike S1+S2 ECD(S-ECD)(D614G) Protein |
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| Catalog No. | RP01291LQ |
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| Description | Recombinant SARS-CoV-2 S1+S2 ECD（S-ECD）(D614G) Protein with His tag is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Gln1208(Asp614Gly)) of sars-cov-2 S1+S2 ECD（S-ECD）(D614G) (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized recombinant SARS-CoV-2 Spike S1+S2 ECD at 2 μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.15-2.58 ng/mL.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 1.0 EU/μg of the protein by LAL method. |
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| Formulation | Supplied as a 0.22 μm filtered solution in PBS, pH 7.4. |
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| Species | SARS-CoV-2 |
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| Calculated MW | 133.52 kDa |
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Product information
| Synonym | Envelope; SARS-CoV-2 Spike RBD (N501Y); Spike; Spike ECD; Spike RBD; Spike S1; Spike S2; Spike S2 ECD; S1-RBD protein; NCP-CoV RBD Protein; novel coronavirus RBD Protein; 2019-nCoV RBD Protein; S glycoprotein Subunit1 RBD Protein |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
