제품 > 항체 > 단일클론항체(mAb)

SARS-CoV-2 Spike RBD Mouse mAb (A23771)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:SARS-CoV-2

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with SARS-CoV-2 Spike RBD using SARS-CoV-2 Spike RBD Mouse mAb (A23771) at 1:500 dilution.
Secondary antibody:HRP Goat Anti-Mouse IgG (H+L)(AS003) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Dot Blot - SARS-CoV-2 Spike RBD Mouse mAb (A23771)

Immobilized Recombinant SARS-CoV-2 Spike RBD Protein (RP01278LQ) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Spike RBD mAb (A23771) with a linear range of 0.78-50ng/mL.

Overview

Product name	SARS-CoV-2 Spike RBD Mouse mAb
Catalog No.	A23771
Host species	Mouse
Purification method	Affinity purification
Isotype	IgG2b, kappa
CloneNo.	AMC0346R

Background

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.

Immunogen information

Immunogen	Recombinant fusion protein of SARS-CoV-2 CoV spike RBD.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	spike glycoprotein; SARS-CoV-2 Spike RBD
Calculated MW
Observed MW	30kDa

Applications

Reactivity	SARS-CoV-2
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 DB 1:500 - 1:2000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	293T
Cellular location

Documents

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Research Area

SARS-CoV-2 Spike RBD Mouse mAb images

Western blot - SARS-CoV-2 Spike RBD Mouse mAb (A23771)

Dot Blot - SARS-CoV-2 Spike RBD Mouse mAb (A23771)

Immobilized Recombinant SARS-CoV-2 Spike RBD Protein (RP01278LQ) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Spike RBD mAb (A23771) with a linear range of 0.78-50ng/mL.

* For research use only. Not for therapeutic or diagnostic purposes.