| Product name | Recombinant SARS-CoV-2 Spike S1(B.1.1.529/Omicron) Protein |
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| Catalog No. | RP02108 |
| Description | Recombinant SARS-CoV-2 Spike S1(B.1.1.529/Omicron) Protein is produced by Expi293 cells expression system. The target protein is expressed with sequence (Gln14-Arg683(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H)) of SARS-COV-2 Spike S1(B.1.1.529/Omicron) (Accession #QHO60594.1) fused with His tag at the C-terminus. |
| Bio-Activity | Immobilized Human ACE2, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for SARS-COV-2 Spike S1 (Omicron B.1.1.529) , His Tag with the EC50 of 0.8μg/ml determined by ELISA. |
| Purity | >95% by SDS-PAGE;> 95% by HPLC |
| Endotoxin | < 1 EU/μg of the protein by LAL method. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Species | SARS-CoV-2 |
| Calculated MW | 76.38 kDa |
| Synonym | S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; novel coronavirus s1 Protein |
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| Expression Host | HEK293 cells |
| Tag | C-His |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
