Overview
| Product name | Recombinant SARS-COV-2 Spike RBD (N501Y, K417N, E484K) Protein |
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| Catalog No. | RP02321 |
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| Description | Recombinant SARS-COV-2 Spike RBD (N501Y, K417N, E484K ) Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Arg319-Phe541(N501Y, K417N, E484K)) of SARS-COV-2 Spike RBD (N501Y, K417N, E484K ) fused with His tag at the C-terminal. |
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| Bio-Activity | Immobilized SARS-CoV-2 Spike RBD (N501Y, K417N, E484K) at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.48 μg/mL determined by ELISA.
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| Purity | > 95% by Tris-Bis PAGE;> 95% by SEC-HPLC |
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| Endotoxin | < 1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | SARS-COV-2 |
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| Calculated MW | 26.2 kDa |
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Product information
| Synonym | S protein RBD; Spike glycoprotein Receptor-binding domain; S glycoprotein RBD; Spike protein RBD |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
