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Recombinant SARS-COV-2 Spike S1+S2 ECD(S-ECD)(D614G) Trimer Protein (RP02327)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant SARS-COV-2 Spike S1+S2 ECD(S-ECD)(D614G) Trimer Protein
Catalog No.RP02327
DescriptionRecombinant SARS-COV-2 Spike S Trimer (D614G) Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Val16-Glu1188(D614G)) of SARS-COV-2 Spike S Trimer (D614G) fused with a His tag and Avi at the C-terminal.
Bio-ActivityImmobilized SARS-COV-2 Spike S (D614G) Trimer, His Tag at 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Human ACE2, Fc Tag with the EC50 of 0.1 μg/mL determined by ELISA.
Purity> 95% by Tris-Bis PAGE;> 95% by SEC-HPLC
Endotoxin< 1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
SpeciesSARS-COV-2
Calculated MW136.7 kDa
SynonymS protein; Spike glycoprotein; S glycoprotein; COVID-19
Expression HostHEK293 cells
TagC-His&Avi
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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