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SARS-CoV-2 Spike S1 mAb (A20022)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:SARS-CoV-2

ABclonal:Western blot - SARS-CoV-2 Spike S1 mAb (A20022)

Western blot analysis of lysates from control 293T and SARS-CoV-2 Spike S1-293T trasfected cells, using SARS-CoV-2 Spike S1 mAb, BSA and glycerol free (A20022) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product name	SARS-CoV-2 Spike S1 mAb
Catalog No.	A20022
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC2373

Background

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.

Immunogen information

Immunogen	Recombinant fusion protein of SARS-CoV-2 Spike S1.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	spike glycoprotein
Calculated MW
Observed MW	110kDa

Applications

Reactivity	SARS-CoV-2
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:10000 IF/ICC 1:200 - 1:800 FC 1:50 - 1:200 ELISA 1:1000 - 1:5000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Flow Cytometry
Positive samples	293T cells transfected with SARS-CoV-2 Spike S1 protein
Cellular location
Customer validation	IF(Mus musculus, Homo sapiens) IHC(Mus musculus) WB(Mus musculus) IHC(Mus musculus) IF(Mus musculus)