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DDIT3/CHOP Rabbit mAb (A20987)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using DDIT3/CHOP Rabbit mAb (A20987) at1:1000 dilution incubated overnight at 4℃. C2C12 and C6 cells were treated by tunicamycin (2 μg/ml) for 8 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - DDIT3/CHOP Rabbit mAb (A20987)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using DDIT3/CHOP Rabbit mAb (A20987) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using DDIT3/CHOP Rabbit mAb (A20987) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product name	DDIT3/CHOP Rabbit mAb
Catalog No.	A20987
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC51429

Background

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DDIT3/CHOP (NP_004074.2).
Sequence	MAAESLPFSFGTLSSWELEAWYEDLQEVLSSDENGGTYVSPPGNEEEESKIFTTLDPASLAWLTEEEPEPAEVTSTSQSPHSPDSSQSSLAQEEEEEDQG
Gene ID
Swiss Prot
Synonyms	CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; AltDDIT3; C/EBPzeta; DDIT3/CHOP
Calculated MW	19kDa
Observed MW	27kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 IHC-P 1:200 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	C2C12 treated by tunicamycin, C6 treated by tunicamycin
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Homo sapiens, Fathead minnow, Mus musculus) IHC(Homo sapiens) WB(Homo sapiens, Mus musculus) IF(Homo sapiens,Mus musculus) IHC(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

DDIT3/CHOP Rabbit mAb images

Western blot - DDIT3/CHOP Rabbit mAb (A20987)

Immunohistochemistry - DDIT3/CHOP Rabbit mAb (A20987)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for DDIT3/CHOP Rabbit mAb (A20987)

Product:

DDIT3/CHOP Rabbit mAb

Journal:

Theranostics

Application:

IF:

11.6

Species:

Homo sapiens

PMID:

37649607

Title:

Oleic acid-induced metastasis of KRAS/p53-mutant colorectal cancer relies on concurrent KRAS activation and IL-8 expression bypassing EGFR activation - PMC

References for DDIT3/CHOP Rabbit mAb (A20987)

Product:

DDIT3/CHOP Rabbit mAb

Journal:

Fish & shellfish immunology

Application:

IF:

4.62

Species:

Fathead minnow

PMID:

37661036

Title:

Chlorpyrifos-mediated mitochondrial calcium overload induces EPC cell apoptosis via ROS/AMPK/ULK1

References for DDIT3/CHOP Rabbit mAb (A20987)

Product:

DDIT3/CHOP Rabbit mAb

Journal:

BMC nephrology

Application:

IF:

2.58

Species:

Mus musculus

PMID:

38053041

Title:

IL-33/ST2L signaling alleviates diabetic nephropathy by regulating endoplasmic reticulum stress and apoptosis - PMC

References for DDIT3/CHOP Rabbit mAb (A20987)

Product:

DDIT3/CHOP Rabbit mAb

Journal:

Environmental toxicology

Application:

IF:

4.1

Species:

Homo sapiens

PMID:

38308464

Title:

Licochalcone A induces endoplasmic reticulum stressmediated apoptosis of endometrial cancer cells via upregulation of GRP78 expression

References for DDIT3/CHOP Rabbit mAb (A20987)

Product:

DDIT3/CHOP Rabbit mAb

Journal:

International immunopharmacology

Application:

IF:

5.71

Species:

Homo sapiens

PMID:

37290325

Title:

Aspirin induces immunogenic cell death and enhances cancer immunotherapy in colorectal cancer

References for DDIT3/CHOP Rabbit mAb (A20987)

Product:

DDIT3/CHOP Rabbit mAb

Journal:

Cell death & disease

Application:

IF:

Species:

Mus musculus

PMID:

38378757

Title:

HRD1-induced TMEM2 ubiquitination promotes ER stress-mediated apoptosis through a non-canonical pathway in intestinal ischemia/reperfusion - PMC

References for DDIT3/CHOP Rabbit mAb (A20987)

Product:

DDIT3/CHOP Rabbit mAb

Journal:

Current pharmaceutical design

Application:

IF:

3.31

Species:

Mus musculus

PMID:

37881071

Title:

Rosuvastatin Improves Endothelial Dysfunction in Diabetes by Normalizing Endoplasmic Reticulum Stress via Calpain-1 Inhibition

References for DDIT3/CHOP Rabbit mAb (A20987)

Product:

DDIT3/CHOP Rabbit mAb

Journal:

Acta pharmaceutica Sinica. B

Application:

IF:

14.5

Species:

Mus musculus

PMID:

38572095

Title:

Eubacterium coprostanoligenes alleviates chemotherapy-induced intestinal mucositis by enhancing intestinal mucus barrier

References for DDIT3/CHOP Rabbit mAb (A20987)

Product:

DDIT3/CHOP Rabbit mAb

Journal:

Biology

Application:

IF:

4.2

Species:

Mus musculus

PMID:

38392333

Title:

Effect of CB1 Receptor Deficiency on Mitochondrial Quality Control Pathways in Gastrocnemius Muscle - PMC

References for DDIT3/CHOP Rabbit mAb (A20987)

Product:

DDIT3/CHOP Rabbit mAb

Journal:

Cell biochemistry and biophysics

Application:

IF:

2.6

Species:

Homo sapiens

PMID:

38816653

Title:

TROP2 promotes the proliferation of triple-negative breast cancer cells via calcium ion-dependent ER stress signaling pathway

References for DDIT3/CHOP Rabbit mAb (A20987)

Product:

DDIT3/CHOP Rabbit mAb

Journal:

Regenerative biomaterials

Application:

IHC, WB

IF:

6.7

Species:

Homo sapiens

PMID:

38799701

Title:

Nano-hydroxyapatite promotes cell apoptosis by co-activating endoplasmic reticulum stress and mitochondria damage to inhibit glioma growth - PMC

References for DDIT3/CHOP Rabbit mAb (A20987)

Product:

DDIT3/CHOP Rabbit mAb

Journal:

Pharmaceutics

Application:

IF:

5.4

Species:

Mus musculus

PMID:

39065567

Title:

IDO1 Inhibitor RY103 Suppresses Trp-GCN2-Mediated Angiogenesis and Counters Immunosuppression in Glioblastoma - PMC

References for DDIT3/CHOP Rabbit mAb (A20987)

Product:

DDIT3/CHOP Rabbit mAb

Journal:

Biochemical and biophysical research communications

Application:

IF, IHC, WB

IF:

3.1

Species:

Homo sapiens,Mus musculus

PMID:

38772213

Title:

Mitigation of ROS-triggered endoplasmic reticulum stress by upregulating Nrf2 retards diabetic nephropathy

References for DDIT3/CHOP Rabbit mAb (A20987)

Product:

DDIT3/CHOP Rabbit mAb

Journal:

Environmental pollution (Barking, Essex : 1987)

Application:

IF:

8.9

Species:

Mus musculus

PMID:

38423272

Title:

Inhalation exposure to polystyrene nanoplastics induces chronic obstructive pulmonary disease-like lung injury in mice through multi-dimensional assessment