제품 > 항체 > 단일클론항체(mAb)

4F2hc/CD98/SLC3A2 Rabbit mAb (A23839)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of various lysates, using 4F2hc/CD98/SLC3A2 Rabbit mAb (A23839) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product name	4F2hc/CD98/SLC3A2 Rabbit mAb
Catalog No.	A23839
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC61478

Background

Predicted to enable amino acid transmembrane transporter activity and double-stranded RNA binding activity. Predicted to be involved in carboxylic acid transport and viral entry into host cell. Predicted to act upstream of or within response to exogenous dsRNA. Located in apical pole of neuron. Is active in synapse. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system gland; and spinal cord. Orthologous to human SLC3A2 (solute carrier family 3 member 2).

Immunogen information

Immunogen	Recombinant Protein corresponding to a sequence within amino acids 105-526 of mouse 4F2hc/CD98/SLC3A2 (P10852).
Sequence	ELPVQRWWHKGALYRIGDLQAFVGRDAGGIAGLKSHLEYLSTLKVKGLVLGPIHKNQKDEINETDLKQINPTLGSQEDFKDLLQSAKKKSIHIILDLTPNYQGQNAWFLPAQADIVATKMKEALSSWLQDGVDGFQFRDVGKLMNAPLYLAEWQNITKNLSEDRLLIAGTESSDLQQIVNILESTSDLLLTSSYLSNSTFTGERTESLVTRFLNATGSQWCSWSVSQAGLLADFIPDHLLRLYQLLLFTLPGTPVFSYGDELGLQGALPGQPAKAPLMPWNESSIFHIPRPVSLNMTVKGQNEDPGSLLTQFRRLSDLRGKERSLLHGDFHALSSSPDLFSYIRHWDQNERYLVVLNFRDSGRSARLGASNLPAGISLPASAKLLLSTDSARQSREEDTSLKLENLSLNPYEGLLLQFPFVA
Gene ID
Swiss Prot
Synonyms	4F2; Cd98; Ly10; Mdu1; 4F2HC; Ly-10; NACAE; Ly-m10; Mgp-2hc; 4F2hc/CD98/SLC3A2
Calculated MW	58KDa
Observed MW	70-100kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Moumse kidney, Mouse brain, Mouse testis, Rat testis, Rat kidney
Cellular location	Cell membrane.
Customer validation	WB(Mus musculus)