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ABCA6 Rabbit pAb (A3191)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ABCA6 Rabbit pAb (A3191)

Western blot analysis of various lysates using (A3191) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Overview

Product nameABCA6 Rabbit pAb
Catalog No.A3191
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable ATPase-coupled transmembrane transporter activity and lipid transporter activity. Predicted to be involved in lipid transport. Predicted to be located in Golgi membrane; nucleoplasm; and plasma membrane. Predicted to be active in intracellular membrane-bounded organelle. Is expressed in tail. Orthologous to human ABCA6 (ATP binding cassette subfamily A member 6).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 690-828 of human ABCA6 (NP_525023.2).
SequenceRLKCAGSSMFLKRRWGLGYHLSLHRNEICNPEQITSFITHHIPDAKLKTENKEKLVYTLPLERTNTFPDLFSDLDKCSDQGVTGYDISMSTLNEVFMKLEGQSTIEQDFEQVEMIRDSESLNEMELAHSSFSEMQTAVS
Gene ID
Swiss Prot
Synonyms6330565N06Rik; ABCA6
Calculated MW44kDa/183kDa
Observed MW185kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse liver, Rat liver
Cellular locationMembrane, Multi-pass membrane protein.
Customer validation

WB(Mus musculus)

Documents

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    ABclonal:Western blot - ABCA6 Rabbit pAb (A3191)}

    Western blot - ABCA6 Rabbit pAb (A3191)

    Western blot analysis of various lysates using (A3191) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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