제품 > 항체 > 다중클론항체(pAb)

ABCB10 Rabbit pAb (A13787)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ABCB10 Rabbit pAb (A13787)

Western blot analysis of various lysates using ABCB10 Rabbit pAb (A13787) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - ABCB10 Rabbit pAb (A13787)

Immunofluorescence analysis of C6 cells using ABCB10 Rabbit pAb (A13787) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ABCB10 Rabbit pAb (A13787)

Immunofluorescence analysis of NIH/3T3 cells using ABCB10 Rabbit pAb (A13787) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameABCB10 Rabbit pAb
Catalog No.A13787
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The function of this mitochondrial protein is unknown.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 230-300 of human ABCB10 (NP_036221.2).
SequenceAIRVYLMQTSGQRIVNRLRTSLFSSILRQEVAFFDKTRTGELINRLSSDTALLGRSVTENLSDGLRAGAQA
Gene ID
Swiss Prot
SynonymsM-ABC2; MTABC2; EST20237; ABCB10
Calculated MW79kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesU-87MG, Raji, Mouse brain, Mouse heart
Cellular locationMitochondrion inner membrane, Multi-pass membrane protein

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ABclonal:Western blot - ABCB10 Rabbit pAb (A13787)}

Western blot - ABCB10 Rabbit pAb (A13787)

Western blot analysis of various lysates using ABCB10 Rabbit pAb (A13787) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - ABCB10 Rabbit pAb (A13787)}

Immunofluorescence - ABCB10 Rabbit pAb (A13787)

Immunofluorescence analysis of C6 cells using ABCB10 Rabbit pAb (A13787) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ABCB10 Rabbit pAb (A13787)}

Immunofluorescence - ABCB10 Rabbit pAb (A13787)

Immunofluorescence analysis of NIH/3T3 cells using ABCB10 Rabbit pAb (A13787) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.