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ABCE1 Rabbit mAb (A9135)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - ABCE1 Rabbit mAb (A9135)

Western blot analysis of extracts of various cell lines, using ABCE1 Rabbit mAb (A9135) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - ABCE1 Rabbit mAb (A9135)

Immunofluorescence analysis of NIH-3T3 cells using ABCE1 Rabbit mAb (A9135) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameABCE1 Rabbit mAb
Catalog No.A9135
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1445
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the OABP subfamily. Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. Two transcript variants encoding the same protein have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 500-599 of human ABCE1 ( NP_002931.2).
SequenceAARVVKRFILHAKKTAFVVEHDFIMATYLADRVIVFDGVPSKNTVANSPQTLLAGMNKFLSQLEITFRRDPNNYRPRINKLNSIKDVEQKKSGNYFFLDD
Gene ID
Swiss Prot
SynonymsRLI; OABP; RLI1; ABC38; RNS4I; RNASEL1; RNASELI
Calculated MW67kDa
Observed MW67kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, HT-29, K-562, Mouse liver, Mouse spleen, Mouse pancreas
Cellular locationCytoplasm, Mitochondrion

Documents

Certificate of Compliance

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    ABclonal:Western blot - ABCE1 Rabbit mAb (A9135)}

    Western blot - ABCE1 Rabbit mAb (A9135)

    Western blot analysis of extracts of various cell lines, using ABCE1 Rabbit mAb (A9135) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunofluorescence - ABCE1 Rabbit mAb (A9135)}

    Immunofluorescence - ABCE1 Rabbit mAb (A9135)

    Immunofluorescence analysis of NIH-3T3 cells using ABCE1 Rabbit mAb (A9135) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (1)

    Secondary Antibodies (26)