제품 > 항체 > 다중클론항체(pAb)

ABF3 Rabbit pAb (A22251)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Arabidopsis thaliana

ABclonal:Western blot - ABF3 Rabbit pAb (A22251)

Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using ABF3 Rabbit pAb (A22251) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

Overview

Product nameABF3 Rabbit pAb
Catalog No.A22251
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Encodes an ABA-responsive element-binding protein with similarity to transcription factors that is expressed in response to stress and abscisic acid.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 260-360 of Arabidopsis thaliana ABF3 (NP_567949.1).
SequenceQQPQQLIQTQERPFPKQTTIAFSNTVDVVNRSQPATQCQEVKPSILGIHNHPMNNNLLQAVDFKTGVTVAAVSPGSQMSPDLTPKSALDASLSPVPYMFGR
Gene ID
Swiss Prot
Synonymsabscisic acid responsive elements-binding factor 3; AtABF3; DC3 PROMOTER-BINDING FACTOR 5; DPBF5; F17I5.200; ABF3
Calculated MW
Observed MW50kDa
ReactivityArabidopsis thaliana
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSeedling, Before inflorescence, Rosette leaf
Cellular location

Documents

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    ABclonal:Western blot - ABF3 Rabbit pAb (A22251)}

    Western blot - ABF3 Rabbit pAb (A22251)

    Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using ABF3 Rabbit pAb (A22251) at 1:500 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 45s.

    * For research use only. Not for therapeutic or diagnostic purposes.