제품 > 항체 > 다중클론항체(pAb)

ABI4 Rabbit pAb (A22239)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Arabidopsis thaliana

Western blot analysis of various tissues from Arabidopsis thaliana, using ABI4 Rabbit pAb (A22239) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product name	ABI4 Rabbit pAb
Catalog No.	A22239
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Encodes a member of the DREB subfamily A-3 of ERF/AP2 transcription factor family (ABI4). The protein contains one AP2 domain. There is only one member in this family. Involved in abscisic acid (ABA) signal transduction, ABA-mediated glucose response, and hexokinase-dependent sugar responses. Acts downstream of GUN1 in retrograde signaling. Expressed most abundantly in developing siliques and to a lesser degree in seedlings.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of arabidopsis thaliana ABI4 (NP_181551.1).
Sequence	MDPLASQHQHNHLEDNNQTLTHNNPQSDSTTDSSTSSAQRKRKGKGGPDNSKFRYRGVRQRSWGKWVAEIREPRKRTRKWLGTFATAEDAARAYDRAAVY
Gene ID
Swiss Prot
Synonyms	ABA INSENSITIVE 4; ABSCISIC ACID-INSENSITIVE PROTEIN 4; ATABI4; GIN6; GLUCOSE INSENSITIVE 6; IMPAIRED SUCROSE INDUCTION 3; ISI3; SALOBRENO 5; SAN5; SIS5; SUCROSE UNCOUPLED 6; SUGAR-INSENSITIVE 5; SUN6; T7M7.16; ABI4
Calculated MW	36kDa
Observed MW	36kDa

Applications

Reactivity	Arabidopsis thaliana
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Seedling, Inflorescences, Before inflorescence, Rosette leaf
Cellular location	apical plasma membrane, basolateral plasma membrane, plasma membrane