제품 > 항체 > 단일클론항체(mAb)

ABflo® 450 Rabbit anti-Human IgD mAb (A26480)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and Human PBMC (right) were surface-stained with ABflo® 450 Rabbit anti-Human IgD mAb (A26480, 5 μl/Test, orange line) or ABflo® 450 Rabbit IgG isotype control (5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 450 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 450 Rabbit anti-Human IgD mAb (A26480, 5 μl/Test, right).

Overview

Product name	ABflo® 450 Rabbit anti-Human IgD mAb
Catalog No.	A26480
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC60051-ABflo450

Background

IgD is the major antigen receptor isotype on the surface of most peripheral B-cells, where it is coexpressed with IgM. The membrane-bound IgD (mIgD) induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 99-384 of human IgD.
Sequence	RWPESPKAQASSVPTAQPQAEGSLAKATTAPATTRNTGRGGEEKKKEKEKEEQEERETKTPECPSHTQPLGVYLLTPAVQDLWLRDKATFTCFVVGSDLKDAHLTWEVAGKVPTGGVEEGLLERHSNGSQSQHSRLTLPRSLWNAGTSVTCTLNHPSLPPQRLMALREPAAQAPVKLSLNLLASSDPPEAASWLLCEVSGFSPPNILLMWLEDQREVNTSGFAPARPPPQPRSTTFWAWSVLRVPAPPSPQPATYTCVVSHEDSRTLLNASRSLEVSYVTDHGPMK
Gene ID
Swiss Prot
Synonyms	IGHD
Calculated MW	47kDa
Observed MW	Refer to figures

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Plasma membrane, Secreted.

Documents

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Research Area

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ABflo® 450 Rabbit anti-Human IgD mAb images

Flow CytoMetry - ABflo® 450 Rabbit anti-Human IgD mAb (A26480)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 450 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 450 Rabbit anti-Human IgD mAb (A26480, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.