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ABflo® 647 Rabbit anti-Human IgD mAb (A23113)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human IgD mAb (A23113)

Flow cytometry:1X10^6 Jurkat cells (negative control, Left) and Human PBMC (Right) were surface-stained with ABflo® 647 Rabbit anti-Human IgD mAb(A23113, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells was used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human IgD mAb (A23113)

Flow cytometry:1X10^6 Human PBMC were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human IgD mAb(A23113, 5 μl/Test, right).

Overview

Product nameABflo® 647 Rabbit anti-Human IgD mAb
Catalog No.A23113
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60051-ABf647
IgD is the major antigen receptor isotype on the surface of most peripheral B-cells, where it is coexpressed with IgM. The membrane-bound IgD (mIgD) induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 99-384 of human IgD
SequenceRWPESPKAQASSVPTAQPQAEGSLAKATTAPATTRNTGRGGEEKKKEKEKEEQEERETKTPECPSHTQPLGVYLLTPAVQDLWLRDKATFTCFVVGSDLKDAHLTWEVAGKVPTGGVEEGLLERHSNGSQSQHSRLTLPRSLWNAGTSVTCTLNHPSLPPQRLMALREPAAQAPVKLSLNLLASSDPPEAASWLLCEVSGFSPPNILLMWLEDQREVNTSGFAPARPPPQPRSTTFWAWSVLRVPAPPSPQPATYTCVVSHEDSRTLLNASRSLEVSYVTDHGPMK
Gene ID
Swiss Prot
Synonyms
Calculated MW42kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationPlasma membrane, Secreted

Documents

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ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human IgD mAb (A23113)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human IgD mAb (A23113)

Flow cytometry:1X10^6 Jurkat cells (negative control, Left) and Human PBMC (Right) were surface-stained with ABflo® 647 Rabbit anti-Human IgD mAb(A23113, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells was used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human IgD mAb (A23113)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human IgD mAb (A23113)

Flow cytometry:1X10^6 Human PBMC were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human IgD mAb(A23113, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (8)

재조합 단백질 (1)

Secondary Antibodies (26)