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ABflo® 488 Rabbit anti-Human CD27 mAb (A22063)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD27 mAb (A22063)

Flow cytometry:1X10^6 HEL cells (negative control, left) and Daudi cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD27 mAb(A22063, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD27 mAb (A22063)

Flow cytometry:1X10^6 Human PBMC were surface-stained with ABflo® 488 Rabbit anti-Human CD27 mAb(A22063, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained Human PBMC was used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD27 mAb (A22063)

Flow cytometry:1X10^6 Human PBMC cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD27 mAb(A22063, 5 μl/Test, right).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD27 mAb (A22063)

Flow cytometry:1X10^6 Human PBMC cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test) or ABflo® 488 Rabbit anti-Human CD27 mAb(A22063, 5 μl/Test).The cells were simultaneously stained with ABflo® 647 Rabbit anti-Human/Monkey CD19 mAb(A23009,5 μl/Test).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD27 mAb (A22063)

Flow cytometry:1X10^6 Monkey PBMC cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test) or ABflo® 488 Rabbit anti-Human CD27 mAb(A22063, 5 μl/Test).The cells were simultaneously stained with ABflo® 647 Rabbit anti-Human/Monkey CD19 mAb(A23009,5 μl/Test).

Overview

Product nameABflo® 488 Rabbit anti-Human CD27 mAb
Catalog No.A22063
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC54542-ABf488
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 21-192 of human CD27 (NP_001233.2).
SequenceTPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVSFSPDHHTRPHCESCRHCNSGLLVRNCTITANAECACRNGWQCRDKECTECDPLPNPSLTARSSQALSPHPQPTHLPYVSEMLEARTAGHMQTLADFRQLPARTLSTHWPPQRSLCSSDFIRI
Gene ID
Swiss Prot
SynonymsT14; S152; Tp55; TNFRSF7; S152. LPFS2
Calculated MW29kDa
Observed MWRefer to figures
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationMembrane, Single-pass type I membrane protein

Documents

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ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD27 mAb (A22063)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD27 mAb (A22063)

Flow cytometry:1X10^6 HEL cells (negative control, left) and Daudi cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD27 mAb(A22063, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD27 mAb (A22063)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD27 mAb (A22063)

Flow cytometry:1X10^6 Human PBMC were surface-stained with ABflo® 488 Rabbit anti-Human CD27 mAb(A22063, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained Human PBMC was used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD27 mAb (A22063)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD27 mAb (A22063)

Flow cytometry:1X10^6 Human PBMC cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD27 mAb(A22063, 5 μl/Test, right).
ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD27 mAb (A22063)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD27 mAb (A22063)

Flow cytometry:1X10^6 Human PBMC cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test) or ABflo® 488 Rabbit anti-Human CD27 mAb(A22063, 5 μl/Test).The cells were simultaneously stained with ABflo® 647 Rabbit anti-Human/Monkey CD19 mAb(A23009,5 μl/Test).
ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD27 mAb (A22063)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD27 mAb (A22063)

Flow cytometry:1X10^6 Monkey PBMC cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test) or ABflo® 488 Rabbit anti-Human CD27 mAb(A22063, 5 μl/Test).The cells were simultaneously stained with ABflo® 647 Rabbit anti-Human/Monkey CD19 mAb(A23009,5 μl/Test).

* For research use only. Not for therapeutic or diagnostic purposes.

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