Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human
Western blot analysis of lysates from HeLa cells, using Phospho-PKR/EIF2AK2-T446 Rabbit mAb (A4869) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
Western blot analysis of lysates from Jurkat cells, using Phospho-PKR/EIF2AK2-T446 Rabbit mAb (A4869) at 1:1000 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
| Product name | Phospho-PKR/EIF2AK2-T446 Rabbit mAb |
|---|---|
| Catalog No. | AP1134 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC0293 |
| Immunogen | A synthetic phosphorylated peptide around T446 of human PKR/EIF2AK2E2AK2 (P19525). |
|---|---|
| Sequence | KRTRS |
| Gene ID | |
| Swiss Prot | |
| Synonyms | PKR; PRKR; DYT33; LEUDEN; EIF2AK1; PPP1R83; Phospho-PKR/EIF2AK2-T446 |
| Calculated MW | 62kDa |
| Observed MW | 74kDa |
| Reactivity | Human |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | HeLa treated by Calyculin A, Jurkat treated by Calyculin A |
| Cellular location | Cytoplasm, Nucleus, perinuclear region. |
| Customer validation | WB(Homo sapiens, Mus musculus, Sus scrofa) IF(Homo sapiens) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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