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Phospho-PKR/EIF2AK2-T446 Rabbit pAb (AP1251)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from NIH/3T3 cells using Phospho-PKR/EIF2AK2-T446 Rabbit pAb (AP1251) at 1:2000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - Phospho-PKR/EIF2AK2-T446 Rabbit pAb (AP1251)

Immunofluorescence analysis of HeLa treated with CA and HeLa cells using Phospho-PKR/EIF2AK2-T446 Rabbit pAb (AP1251) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Phospho-PKR/EIF2AK2-T446 Rabbit pAb
Catalog No.	AP1251
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T446 of human PKR/EIF2AK2 (NP_002750.1).
Sequence	KRTRSK
Gene ID
Swiss Prot
Synonyms	PKR; PRKR; DYT33; LEUDEN; EIF2AK1; PPP1R83; Phospho-PKR/EIF2AK2-T446
Calculated MW	62kDa
Observed MW	68kDa/

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	NIH/3T3 treated by Calyculin A
Cellular location	Cytoplasm, Nucleus, perinuclear region.
Customer validation	WB(Mus musculus)