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PKR/EIF2AK2 Rabbit pAb (A4047)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - PKR/EIF2AK2 Rabbit pAb (A4047)

Western blot analysis of lysates from A-549 cells, using PKR/EIF2AK2 Rabbit pAb (A4047) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - PKR/EIF2AK2 Rabbit pAb (A4047)

Immunohistochemistry analysis of paraffin-embedded human liver using PKR/EIF2AK2 Rabbit pAb (A4047) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - PKR/EIF2AK2 Rabbit pAb (A4047)

Immunofluorescence analysis of U2OS cells using PKR/EIF2AK2 Rabbit pAb (A4047) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePKR/EIF2AK2 Rabbit pAb
Catalog No.A4047
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PKR/EIF2AK2 (NP_002750.1).
SequenceMAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEI
Gene ID
Swiss Prot
SynonymsPKR; PRKR; DYT33; LEUDEN; EIF2AK1; PPP1R83; PKR/EIF2AK2
Calculated MW62kDa
Observed MW72kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-549
Cellular locationCytoplasm, Nucleus, perinuclear region
Customer validation

WB(Homo sapiens)

IF(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - PKR/EIF2AK2 Rabbit pAb (A4047)}

Western blot - PKR/EIF2AK2 Rabbit pAb (A4047)

Western blot analysis of lysates from A-549 cells, using PKR/EIF2AK2 Rabbit pAb (A4047) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - PKR/EIF2AK2 Rabbit pAb (A4047)}

Immunohistochemistry - PKR/EIF2AK2 Rabbit pAb (A4047)

Immunohistochemistry analysis of paraffin-embedded human liver using PKR/EIF2AK2 Rabbit pAb (A4047) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - PKR/EIF2AK2 Rabbit pAb (A4047)}

Immunofluorescence - PKR/EIF2AK2 Rabbit pAb (A4047)

Immunofluorescence analysis of U2OS cells using PKR/EIF2AK2 Rabbit pAb (A4047) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (5)

Secondary Antibodies (26)