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ABflo® 488 Rabbit anti-Human ERG mAb (A22496)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human ERG mAb (A22496)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and MOLT-4 cells (right) were intracellularly-stained with ABflo® 488 Rabbit anti-Human ERG mAb (A22496, 2 μg/mL, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 2 μg/mL, blue line). Non-fluorescently stained cells was used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human ERG mAb (A22496)

Flow cytometry: 1X10^6 MOLT-4 cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human ERG mAb (A22496, 5 μl/Test, right).

Overview

Product nameABflo® 488 Rabbit anti-Human ERG mAb
Catalog No.A22496
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC56338-ABf488
This gene encodes a member of the erythroblast transformation-specific (ETS) family of transcriptions factors. All members of this family are key regulators of embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. The protein encoded by this gene is mainly expressed in the nucleus. It contains an ETS DNA-binding domain and a PNT (pointed) domain which is implicated in the self-association of chimeric oncoproteins. This protein is required for platelet adhesion to the subendothelium, inducing vascular cell remodeling. It also regulates hematopoesis, and the differentiation and maturation of megakaryocytic cells. This gene is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia. More than two dozens of transcript variants generated from combinatorial usage of three alternative promoters and multiple alternative splicing events have been reported, but the full-length nature of many of these variants has not been determined.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human ERG (NP_891548.1).
SequenceMASTIKEALSVVSEDQSLFECAYGTPHLAKTEMTASSSSDYGQTSKMSPRVPQQDWLSQPPARVTIKMECNPSQVNGSRNSPDECSVAKGGKMVGSPDTV
Gene ID
Swiss Prot
Synonymsp55; erg-3
Calculated MW54kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC (intra) 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application
Positive samples
Cellular locationCytoplasm, Nucleus

Documents

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ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human ERG mAb (A22496)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human ERG mAb (A22496)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and MOLT-4 cells (right) were intracellularly-stained with ABflo® 488 Rabbit anti-Human ERG mAb (A22496, 2 μg/mL, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 2 μg/mL, blue line). Non-fluorescently stained cells was used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human ERG mAb (A22496)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human ERG mAb (A22496)

Flow cytometry: 1X10^6 MOLT-4 cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human ERG mAb (A22496, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

Secondary Antibodies (26)